Removing hard disk from a virtual machine

Hello,

I have added hard disks to many virtual machines but never in a situation where we need to remove them.  For example below with a virtual machine named "exchange1" with 3 disks.  I need to remove hard disk3.  The server admin will remove the drive from the Windows machine.  Once that is done, I just remove hard disk3.  The disks are from different datastores.  Is there anything else I have to consider?  This can be done when the machine is on?  Also, the reclaim disk space will occur right away?

Hard disk1  (thin provision), Windows C drive

Hard disk2 (thin provision),   Windows E drive

Hard disk3 (thin provision), Windows F drive

Thanks,
TT